Validation of Hemolysis Index Thresholds Optimizes Detection of Clinically Significant Hemolysis

Mechanism of Hemolysis by Complement

1. Sensitization confers upon the red cell the property of adsorbing complement from solution. The submicroscopic film of immune serum protein deposited upon the cell surface during sensitization, and completely analogous to the precipitate formed in a soluble antigen-antibody reaction (e.g., sheep serum vs. rabbit anti-sheep serum) acts as absorbent, the degree of sensitization (size of the fi...

Relation of methemoglobin to hemolysis.

Hemolytic index – A tool to measure hemolysis in vitro

Introduction: Hemolysis is the commonest causeof pre analytical error. Hemoglobin released interferes with analyte concentration chemically and optically.

Microscopic detection of mixed malarial infections: improvement by saponin hemolysis.

OBJECTIVE The objective of the present study was to determine whether saponin hemolysis could improve microscopic detection of malaria parasites in human blood, since it has been previously reported that the technique has been used to enrich Plasmodium falciparum culture to >or=90% parasitemia. MATERIAL AND METHODS Blood samples from suspected malaria cases were first examined in routine thic...

Preferential Hemolysis of Immature Erythrocytes

The findings indicate that experimental iron deficiency anemia is associated with hemolysis of red cells of various ages, but with preferential destruction of the youngest cells. Degradation of hemoglobin from reticulocytes is sufficient to account for a major fraction of the increase in erythropoietic bilirubin production found in this disorder, as has also been shown for physiologically regul...